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KMID : 0903520050480040364
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.364 ~ p.369
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Identification and Growth Activity to Bifidobacterium spp. of Locust Bean Gum Hydrolysates by Trichoderma harzianum β-mannanase
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Kim Yu-Jin
Park Gwi-Gun
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Abstract
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This study was performed to elucidate substrate specificity to the locust bean gum galactomannan by Trichoderma harzianum β-mannanase. The medium composition for enzyme production were determined 3% cellulose, 3% corn steep liquor, 1% KH2PO4, 0.2% (NH4)2SO4, and incubated for 115 hr at 28oC. The β-mannanase exhibited maximum activity at pH 4.5 and 60oC. Locust bean gum galactomannan was hydrolyzed by the β-mannanase, and then hydrolysates separated by activated carbon column chromatography. The main hydrolysates were composed of D.P 4 and 7 galactosyl mannooligosaccharides by TLC. For the elucidate the structure of D.P 4 and 7 oligosaccharides, methylation analysis was performed. D.P 4 and 7 were identified as M-M-M-M and M-M- M-M-M (G- and M-represent α-1,6-D-galactosidic and β-1,4-mannosidic linkages, respectively).
G-----G
To investigate the effects of locust bean gum galactosyl mannooligosaccharides on the in vitro growth of B. longum, B. bifidum, B. infantis, and B. breve, Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon source such as D.P 4 and 7 galactosyl mannooligosaccharides, respectively. B. longum grew up 3.4-fold and 4.3-fold more effectively by the replacement of D.P 4 and 7 galactosyl mannooligosaccharides as the carbon source in a comparasion of standard MRS.
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KEYWORD
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β-Mannanase, Trichoderma harzianum, locust bean gum
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